RFX5 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, EMSA, IP, WB
Recommend Dilution	Western blotting: 1/250. Immunoprecipitation. ELISA. Electrophoretic Mobility Shift Assay: using 0.5 to 1.0 µl per assay. Recommended Dilutions: This product was assayed by immunoblot and found to be reactive against RFX5 (C-terminal specific) from a variety of fibroblast and B-cell lysates at a dilution of 1:250 to 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-RFX5 (C-terminal specific) is suitable for the detection by immunoblot of human RFX5 a shows a 75 kDa band. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 µl per assay.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	RFX5 (C-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity	Western blot of RFX5 shows a 75 kDa band. A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.
Formulation	0.01M Na phosphate, 0.15M NaCl, pH7.4 with 0.02% Sodium azide as preservative. State: Ig Fraction State: Liquid (sterile filtered) purified Ig fraction .
Concentration	lot specific
Purification	Prepared from monospecific antiserum by delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Gene Name	regulatory factor X5
Database Link	Entrez Gene 5993 Human UniProt ID P48382
Background	Human RFX5 from a variety of fibroblast and B-cell lysates. Recognition is at the C-terminal region. RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.
Synonyms	Regulatory factor X 5
Reference Data

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