Midkine (MDK) Rabbit Polyclonal Antibody
CAT#: PP1213P1
Midkine (MDK) rabbit polyclonal antibody, Aff - Purified
Conjugation: Biotin
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CNY 3,910.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | Neutralization. ELISA: To detect hMidkine by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (>98%) recombinant hMidkine |
Formulation | 0.5 X PBS pH 7.4 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore in sterile water to a concentration of > 0.2 mg/ml. |
Purification | Affinity chromatography employing immobilized hMidkine matrix. |
Conjugation | Unconjugated |
Storage Condition | Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | midkine (neurite growth-promoting factor 2) |
Database Link | |
Background | Midkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines. |
Synonyms | MK, MDK, MK1, NEGF2, Neurite outgrowth-promoting protein |
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