GRO beta (CXCL2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization (To yield one-half maximal inhibition [ND50] of the biological activity of hGRO-beta (100.00 ng/ml), a concentration of 0.5-1.0 µg/ml of this antibody has been used with previous lots) Indirect ELISA To detect hGRO-beta by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hGRO-beta. Sandwich ELISA: To detect hGRO-beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with biotinylated anti-Human GRO-beta as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO-beta. Western Blot To detect hGRO-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant hGRO-beta (human GRO-beta)
Specificity	This antibody detects GRO-beta.
Formulation	PBS, pH 7.2 State: Aff - Purified State: Sterile filtered fraction, lyophilized
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized hGRO-beta matrix.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 2
Database Link	Entrez Gene 2920 Human UniProt ID P19875
Background	GRO beta is a member of the CXC, or chemokine class. It contains the ELR domain immediately preceding the first cysteine residue near the amino terminus. Other chemokines in this group include IL8, GRO alpha/beta/gamma, mouse KC, ENA78, GCP2, PBP/CTAPIII/beta TG/NAP2. These chemokines act primarily on neutrophils as chemoattractants and activators, including neutrophil degradation with release of myloperoxidase and other enzymes. GRO beta was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. GRO beta is an approximately 8 kDa polypeptide of 73 amino acids. The precursor form of GRO beta consists of 100 amino acids. To generate the mature GRO beta, the precursor cleaves its amino terminal 27 amino acids. GRO beta shows 60% amino acid homology to human GRO alpha and GRO gamma.
Synonyms	C-X-C motif chemokine 2, GRO2, GROB, MIP-2A, SCYB2, Gro-beta, MIP2-alpha
Note	Centrifuge vial prior to opening.
Reference Data

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