GDNF Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Direct ELISA: To detect Human GDNF by direct ELISA (using 100 ml/well antibody solution) this GDNF antibody can be used at a concentration of ~1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 2000-4000 pg/well of recombinant Human GDNF. Sandwich ELISA: To detect Human GDNF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human GDNF (Cat.-No PP1010P) as a capture antibody, allows the detection of at least 2000–4000 pg/well of recombinant Human GDNF. Western Blot: To detect hGDNF by Western Blot analysis this GDNF antibody can be used at a concentration of 0.1-0.2 mg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human GDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	E.coli-derived, 30.4 kDa Recombinant Human GDNF (Cat.-No PA059)
Specificity	Human Glial Derived Neurotrophic Factor.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography.
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Predicted Protein Size	~150 kDa
Gene Name	glial cell derived neurotrophic factor
Database Link	Entrez Gene 2668 Human UniProt ID P39905
Background	Glial cell line derived neurotrophic factor (GDNF) is a highly conserved neurotrophic factor that is a distant member of the TGF beta superfamily. The GDNF gene product is processed to a disulphide-linked homodimer, which is a ligand for the RET protooncogene. GDNF protects and repairs dopamine-containing neurons, which degenerate in Parkinson's disease, and motor neurons, which die in amyotrophic lateral sclerosis. The use of GDNF in the treatment of Parkinson's disease has shown promise in the clinic. Treatment of spinal cord injuries with GDNF has also produced neurological improvement.
Synonyms	Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor, ATF
Note	Centrifuge vial prior to opening!
Reference Data

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