Ifng Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Sandwich ELISA: To detect Rat IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IFN-γ (Cat.-No ) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IFN-γ. Western blot: To detect Rat IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IFN-γ a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of Rat IFN-γ (0.3 ng/ml), a concentration of 60.0-65.0 ng/ml of this antibody is required. Immunohistochemistry: Use 1 µg/ml as a starting dilution. Antigen retrieval may be necessary for paraffin sections.
Reactivity	Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Highly purified E.coli derived recombinant Rat Inferferon gamma (Cat.-No PA036)
Specificity	This antibody recognises Rat Interferon gamma.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized Rat IFN-γ matrix.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	interferon gamma
Database Link	Entrez Gene 25712 Rat UniProt ID P01581
Background	Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
Synonyms	IFN-gamma, gamma IFN
Note	Centrifuge vial prior to opening!
Reference Data

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