MCP1 (CCL2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Murine JE (MCP-1) by Direct ELISA (using 100 µl/well) solution) a concentration of ~1.0 µg/ml of this antibody is required. This antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Murine JE(MCP-1). Sandwich ELISA: To detect Murine JE (MCP-1) by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Purified Anti-Murine JE (MCP-1)(PP020P1, PP020P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine JE(MCP-1). Western Blot: To detect Murine JE(MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine JE(MCP-1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Mouse JE (MCP-1).
Specificity	This antibody reacts with Murine JE (MCP-1).
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 2
Database Link	UniProt ID P13500
Background	This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Synonyms	C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11
Note	Centrifuge vial prior to opening!
Reference Data

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