CXCL7 (PPBP) Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: This antibody can be used at a concentration of 2-4 µg/ml, as a capture antibody in in combination with antigen affinity purified anti-human NAP-2 (Cat.-No PP1056B) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. Western Blot: To detect hNAP-2 this antibody can be used at a concentration of 0.5-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	E.coli derived Recombinant Human NAP-2 (CXCL7).
Specificity	Recognises Human NAP-2.  Other species not tested.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified Ig fraction from Cell Culture
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Ammonium Sulfate co-Precipitation
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	pro-platelet basic protein
Database Link	Entrez Gene 5473 Human UniProt ID P02775
Background	NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
Synonyms	CTAP3, SCYB7, TGB1, THBGB1
Reference Data

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