Interferon beta (IFNB1) Rat Monoclonal Antibody [Clone ID: RMMB-1]

Specifications

Product Data
Clone Name	RMMB-1
Applications	ELISA, FN
Recommend Dilution	Direct Binding ELISA.  Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of mouse interferon beta (Mu-IFN-β) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Mu-IFN-β provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for effective neutralization of Mu-IFN-β.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse Interferon Beta
Specificity	Rat Monoclonal Antibody against Mouse Interferon Beta.  Bioactivity: Weakly neutralizes mouse interferon beta, but not recommended for neutralization assays (for this application products 32400-1 or 32401-1 are recommended); binds to mouse interferon beta.
Formulation	PBS) containing 0.1% bovine serum albumin (BSA) State: Purified
Conjugation	Unconjugated
Gene Name	interferon beta 1
Database Link	Entrez Gene 15977 Mouse UniProt ID P01574
Synonyms	IFN-beta, IFNB1, IFB, Fibroblast interferon
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway

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