ISG15 Mouse Monoclonal Antibody [Clone ID: 2.1]

Specifications

Product Data
Clone Name	2.1
Applications	WB
Recommend Dilution	Western blot. Suggested concentration: In our hands good visualization of ISGylated proteins was obtained using an antibody concentration of 1 μg/ml; however, 0.1 μg/ml or lower can be used to give reasonable visualization of the free ISG band. Optimal dilutions should be determined by each laboratory for each application.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human ISG15
Specificity	Detects Human ISG15.  Detects conjugated and free ISG15. Greater than 95% purity.  No cross-reactivity observed with ubiquitin on Western Blot.
Formulation	50mM Tris HCl, 75mM NaCl, 50% Glycerol pH 8 State: Purified State: Liquid
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	After receipt, this product should be kept at -20°C for retention of full activity. Do not store below -20°C.
Gene Name	ISG15 ubiquitin-like modifier
Database Link	Entrez Gene 9636 Human UniProt ID P05161
Background	Many published reports indicate that ISG15 (G1P2) is commonly upregulated in response to Type I interferon stimulation. Though a wide range of cellular activities are influenced by ISG15 expression including Type I interferon signaling, translation, chromatin remodeling, cell motility, protein trafficking, and protein conjugation (ISGylation), the complete spectrum of ISG15-dependent biological sequelae remains to be fully elucidated. Further characterization of new ISG15 target proteins and the role of free ISG15 may offer new insights into mechanistic and immunotherapeutic approaches to human diseases.
Synonyms	UCRP
Reference Data

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