PDYN Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Immunohistochemistry 1:250-1:1000* Dilutions listed as a recommendation. Optimal dilution should be determined by investigator. Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Note: Sodium azide (NaN3) interferes with peroxidase reactions and should not be used with peroxidase methodologies. If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azide-free buffer before performing the peroxidase reaction.
Reactivity	Rat
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Corresponding to residues 235-248 of the rat proDynorphin.
Formulation	PBS State: Serum State: Liquid Affinity Purified Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Background	Dynorphin peptides are members of the opioid peptide family and contain the canonical opioid sequence Tyr-Gly-Gly-Phe. These peptides are derived from prodynorphin and include dynorphin A, dynorphin B and alpha neo-endorphin. Of the opioid peptides, the dynorphins have the highest affinity for the kappa opioid receptor. They are found distributed throughout the nervous system and play a role in many physiological functions.
Synonyms	Proenkephalin-B, Beta-neoendorphin-dynorphin, Preprodynorphin, Prodynorphin, Alpha-neoendorphin,Beta-neoendorphin, Big dynorphin, Dynorphin A, Leu-enkephalin, Rimorphin, Leumorphin, PENKB
Reference Data

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