VMAT2 (SLC18A2) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot (1/500). Immunohistochemistry on Frozen Sections: 1/2000-1/3000 using FITC, with over-night incubation at 2-8°C. Positive Control: Frozen sections of Rat small intestine.
Reactivity	Human, Mouse, Porcine, Rat
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic peptide from the C-terminal of Rat VMAT2 conjugated to BSA.
Specificity	This antiserum recognizes monoaminergic neurons of the CNS, the ECL-cells of the stomach, as well as enteric nerve fibers. In Western blot experiments using a crude synaptic vesicle fraction of Rat brain, the 70 kDa of anti-VMaT2 is recognized (Recommended Dilution: 1/500). Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.
Formulation	State: Serum State: Lyophilized Serum
Reconstitution Method	Dissolve in 50-100 µl distilled water, and dilute further with 0.1M PBS with 1% BSA and 0.09% Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquot) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	solute carrier family 18 member A2
Database Link	Entrez Gene 6571 Human UniProt ID Q05940
Background	The vesicular monoamine transporter is responsible for the vesicular uptake of monoamines, like dopamine, norepinephrine, epinephrine, serotonin and histamine.
Synonyms	SVMT, VAT2
Reference Data

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