Histidine decarboxylase (HDC) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunocytochemistry: 1/1000-1/1500 with overnight incubation at 2-8°C. Immunohistochemistry on Frozen Sections: 1/2,000-1/5,000. Immunohistochemistry on Paraffin Embedded Tissues: 1/2,000-1/5,000 (microwave treatment recommended) Positive Control: Stefanini-fixed frozen sections of Rat fundus. Western blot: 1/1,000-1/5,000. The antiserum detects the 54 kDa and 73 kDa forms in addition to a 63 kDa form (Rat stomach - See Dartsch et al., 1998).
Reactivity	Canine, Guinea Pig, Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Histidine Decarboxylase produced in E.coli.
Specificity	This antibody reacts with HDC. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.
Formulation	State: Serum State: Undiluted Lyophilized Serum
Reconstitution Method	Restore in 100 µl distilled water
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	histidine decarboxylase
Database Link	Entrez Gene 3067 Human UniProt ID P19113
Background	Histidine decarboxylase(HDC) is the enzyme that calalyses the conversion of histidine into histamine. It can be found in the histamine secreting ECL cells of some species, as well as in the mast cells.The biogenic amine histamine is an important modulator of numerous physiological processes, including neurotransmission, gastric acid secretion and smooth muscle tone.
Synonyms	HDC
Reference Data

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