VEGF Receptor 2 (KDR) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, FN, IF, IP, WB
Recommend Dilution	ELISA (5-15 µg/ml). Western blotting (10-20 µg/ml). Immunoprecipitation (1-2 µg/mg protein lysate). FACS (1-5 µg/ml). Neutralizing experiments: Depending on the conditions and ligand concentration start with 25-50 µg/ml. Immunohistochemistry on Paraffin Sections (2-10 µg/ml).
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Human soluble extracellular domain of KDR protein (D1-7).
Specificity	The polyclonal antibody will detect native and recombinant Human VEGFR-2/KDR in ELISA experiments and on the surface or solubilized from different Human cell types.
Formulation	PBS, pH 7.4 without preservatives or stabilizers State: Aff - Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0-1-1.0 mg/ml.
Purification	Antigen Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	The lyophilized antibody, thought stable at room temperature, is best stored desiccated below 0°C. Reconstituted anti-VEGFR-2/KDR is stable at 2-8°C for one month or can be stored in working aliquots at -20°C for more than six months. Avoid repeated freezing and thawing.
Gene Name	kinase insert domain receptor
Database Link	Entrez Gene 3791 Human UniProt ID P35968
Background	VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Synonyms	VEGFR2, FLK1, KDR, VEGF Receptor 2
Reference Data

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