FGF2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of human FGF-2 basic (5 ng/ml) on HUVEC cells, a concentration of about 3.5 µg/ml of this antibody is required. Western Blot: 2-5 µg/ml.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) recombinant Human FGF-2 (basic) [Ala136 – Ser288] produced in E.coli (Cat.-No DA3503XC).
Specificity	The antibody will detect Human FGF-2 (basic).
Formulation	PBS, pH 7.4 without stabilizer or preservative State: Azide Free State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	fibroblast growth factor 2
Database Link	Entrez Gene 2247 Human UniProt ID P09038
Background	FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Synonyms	FGFB, Heparin-binding growth factor 2, Fibroblast growth factor 2 (basic), BFGF, HBGF-2, HBGF2
Reference Data

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