E2F1 Mouse Monoclonal Antibody [Clone ID: KH95]

Specifications

Product Data
Clone Name	KH95
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABC method for 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil, Breast carcinoma.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	BALB/C mice were immunized with a recombinant Human E2F-1 protein.
Specificity	This antibody reacts with a protein of 60 kD. Cellular Localization: Nuclear.
Formulation	State: Purified State: Purified Ascites with Sodium Azide as preservative.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	E2F transcription factor 1
Database Link	Entrez Gene 1869 Human UniProt ID Q01094
Background	E2Fs are DNA-binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis.
Synonyms	E2F-1, RBBP3, PBR3
Reference Data

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