ERCC1 Mouse Monoclonal Antibody [Clone ID: 8F1]
CAT#: DM336-05
ERCC1 mouse monoclonal antibody, clone 8F1, Purified
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CNY 7,369.00
货期*
4周
规格
Specifications
Product Data | |
Clone Name | 8F1 |
Applications | IHC, IP, WB |
Recommend Dilution | Immunoprecipitation. Immunohistochemistry on Paraffin Embedded Sections: 1/100-1/200 in an ABC method. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to Immunostaining. Incubation Time: 30 minutes at RT. Recommended Positive Control: Tonsil. Western Blot: 1/100-1/500. use Human lung cell line as Positive Control. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | BALB/C mice were injected with full length recombinant human ERCC1 protein. |
Specificity | This antibody reacts with a 33-36 kDa protein known as ERCC1 polypeptide. Cellular localization: Nuclear. |
Formulation | State: Purified State: Liquid purified IgG fraction Preservative: < 0.1% Sodium Azide |
Purification | Protein A Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store the antibody undiluted at 2-8°C. |
Gene Name | ERCC excision repair 1, endonuclease non-catalytic subunit |
Database Link | |
Background | The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells. |
Synonyms | DNA excision repair protein ERCC-1, COFS4, UV20 |
Note | Molecular weight of antigen :33-36 kDa |
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