Heparan Sulfate Proteoglycan 2 (HSPG2) Rat Monoclonal Antibody [Clone ID: A7L6]

Specifications

Product Data
Clone Name	A7L6
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Immunoblotting: 1/100-1/1000. Immunocytochemistry. Immunohistochemistry on Frozen and Paraffin-Embedded Tissues: 1/25-1/200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Reactivity	Bovine, Human, Mouse, Rat, Cod, Wolffish
Host	Rat
Clonality	Monoclonal
Immunogen	High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
Specificity	A7L6 recognizes domain IV of the core protein of the large Heparan Sulphate Proteoglycan or Perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to Heparitinase treatment.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	heparan sulfate proteoglycan 2
Database Link	Entrez Gene 3339 Human UniProt ID P98160
Background	Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.
Synonyms	PLC, Basement membrane-specific heparan sulfate proteoglycan core protein
Reference Data

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