MYOD1 Mouse Monoclonal Antibody [Clone ID: 5.2F]

Specifications

Product Data
Clone Name	5.2F
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	ELISA. Western Blot: 1 µg/ml. Immunofluorescence. Electron microscopy. Immunoprecipitation: 2 µg/mg of protein lysate. Immunohistochemistry on Frozen and Paraffin Sections: 2-4 µg/ml.
Reactivity	Chicken, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.
Specificity	This antibody detects MyoD1.
Formulation	Phosphate buffered saline with 0.08% Sodium Azide as preserative State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	myogenic differentiation 1
Database Link	Entrez Gene 17927 Mouse UniProt ID P10085
Background	Recognizes a phosphoprotein of 45kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.
Synonyms	MYF3, MYOD, Myoblast determination protein 1, Myogenic factor 3, BHLHC1
Reference Data

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