Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: Ks19.1 (A53-B/A2)]

Specifications

Product Data
Clone Name	Ks19.1 (A53-B/A2)
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	ELISA. Western blot. Immunofluorescence. Flow Cytometry. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 1/100-1/200. Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Recommended Positive Control: Apendix
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human breast cancer cell line MCF-7
Specificity	Specific for the rod domain (aa 312-335) of Cytokeratin 19 (40 kDa) in Human tissue. Clone A53-B/A2 detects a band of ~45 kDa in Western blotting using an A-549 Human alveolar adenocarcinoma cell line lysate.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Tissue Culture Supernatant Preservative: 15mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 19
Database Link	Entrez Gene 3880 Human UniProt ID P08727
Background	This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours. Keratin 19 is often co-expressed with keratin 7.
Synonyms	Cytokeratin-19, Keratin-19, KRT19, CK19, K19
Reference Data

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