MCP1 (CCL2) Mouse Monoclonal Antibody [Clone ID: S101]

Specifications

Product Data
Clone Name	S101
Applications	ELISA
Recommend Dilution	ELISA: This MCP1 antibody HRP-conjugated can be used as a Tracer/Detection antibody in Sandwich ELISA applications for Human MCP-1 detection in combination with a Capture antibody Clone S14 (Cat.-No DM2032) Suggested Capture Coating Dose: ~0.3 µg/ml Substrate: TMB. If the above suggested conditions are followed approximately 1.5 pg/mL of MCP-1 in serum/plasma or 4 pg/ml of MCP-1 in medium can be detected with an assay range of 0-1600 pg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant Human MCP1.
Specificity	This monoclonal antibody reacts with natural and recombinant Human MCP-1. Does not react with Human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1 beta (IL1 beta), serum amyloid A (SAA) and epidermal growth factor (EGF)
Formulation	0.01M PBS, pH 7.0 ± 0.1 in 50% Glycerol and 0.01% Thimerosal as a bacteriostat Label: HRP State: Liquid purified IgG fraction Label: Horseradish Peroxidase
Purification	Affinity Chromatography on Protein G
Conjugation	HRP
Storage Condition	Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 2
Database Link	Entrez Gene 6347 Human UniProt ID P13500
Background	Monocyte chemotactic protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or beta) chemokines (1-4). The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis (5), rheumatoid arthritis (6) and atherosclerosis (7). MCP-1 may also contribute to tumor progression and angiogenesis (8). Signaling by MCP-1 is mediated by the G-protein coupled receptor CCR2 (9).
Synonyms	C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway

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