MCP1 (CCL2) Mouse Monoclonal Antibody [Clone ID: S14]

Specifications

Product Data
Clone Name	S14
Applications	ELISA, FN, WB
Recommend Dilution	ELISA: React with Human MCP-1. This antibody can be used as a Capture antibody in Sandwich ELISA applications for Human MCP-1 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated Cat.-No DM2034HRP or Biotin conjugated Cat.-No DM2034B) Suggested Capture Coating Dose: 0.3 µg/well. Substrate: TMB. If the above suggested conditions are followed approximately 1.5 pg/mL of MCP-1 in serum/plasma or 4 pg/mL of MCP-1 in medium can be detected with an assay range of 0-1600 pg/mL. Neutralizing: The antibody (Clone S14) at a concentration of 0.5 μg/ml, neutralized 1nM recombinant Human MCP-1 induced monocyte chemotaxis in blind well chemotaxis chambers. It was also found that S-14 could inhibit the activity of native MCP-1 at concentrations similar to inhibitory doses for recombinant MCP-1. Western Blot: MCP1 antibody at 0.1-1.0 µg/mL will allow visualization of 0.1 µg/lane of Recombinant Human MCP1.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant Human MCP1.
Specificity	This MCP1 antibody reacts with natural and recombinant Human MCP-1. Does not react with Human Interleukin-8 (IL-8) and other Human cytokines tested such as Interleukin-1 beta (IL1 beta), Serum Amyloid A (SAA) and Epidermal Growth Factor (EGF).
Formulation	0.01M PBS, pH 7.2 without preservatives State: Azide Free State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with double distillated water to adjust the final concentration to 1.00 mg/ml
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 2
Database Link	Entrez Gene 6347 Human UniProt ID P13500
Background	This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Synonyms	C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway

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