Cytokeratin 8 (KRT8) (+ KRT18) Mouse Monoclonal Antibody [Clone ID: 5D3]

Specifications

Product Data
Clone Name	5D3
Applications	IHC, WB
Recommend Dilution	Cell Suspensions. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (After Microwave treatment, Proteolytic treatment optional). Immunoblotting (Western): Clone NCL5D3 is directed against the the 52.2 kDa keratin K8 (originally reported also to 45 kDa keratin K18; formerly also designated cytokeratins according to the Moll-catalogue). Working Dilution: 1/5-1/10, dilute immediately before use. Dilution Buffer: PBS with 1% BSA and 0.09% Sodium Azide. Recommended Positive Control: Glandular epithelium.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Isolated from human breast cancer carcinoma cell line MCF-7. Epitope has been mapped to aa 353-367 on alpha helical rod domain of keratin K8 (See Waseem et al., 2004).
Specificity	The antibody recognizes all simple epithelia including glandular epithelia. It does not react with stratified squamous epithelium. Useful for the differentiation between carcinomas and lymphomas.
Formulation	State: Ascites State: Liquid Ascites Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 8
Database Link	Entrez Gene 3856 Human UniProt ID P05787
Background	Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with Cytokeratin 18 (type A; acidic subfamily of low molecular weight keratins).
Synonyms	KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A
Reference Data
Protein Families	Druggable Genome

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