Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: RCK108]

Specifications

Product Data
Clone Name	RCK108
Applications	IF, IHC, WB
Recommend Dilution	Western Blot. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: No proteolytic pretreatment required, Optimal results are obtained when using the microwave retrieval method in citrate buffer. Working Dilution: 1/10-1/20 (ABC Method) for Immunohistochemistry. Dilution Buffer: Dilute immediately with PBS or TBS. Positive Control: Glandular epithelia.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human bladder cell line T24
Specificity	This antibody specifically reacts with keratin K19 (formerly also designated cytokeratin 19; 40 kD protein in immunoblotting), found in most glandular simple type epithelia and tumors derived therefrom (adenocarcinoma), most squamous cell carcinoma.
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant containing 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 19
Database Link	Entrez Gene 3880 Human UniProt ID P08727
Background	Keratin-19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours. Keratin 19 is often co-expressed with keratin 7.
Synonyms	Cytokeratin-19, Keratin-19, KRT19, CK19, K19
Reference Data

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