Clostridium botulinum Toxin B Mouse Monoclonal Antibody [Clone ID: GR3G7]

Specifications

Product Data
Clone Name	GR3G7
Applications	ELISA, FC, WB
Recommend Dilution	Flow cytometry: 1.2 µg/106 cells. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
Reactivity	Clostridium botulinum
Host	Mouse
Clonality	Monoclonal
Immunogen	Genetic immunisation with cDNA encoding BoNT/B.
Specificity	Recognizes Botulinum Neurotoxin type B (BoNT/B).
Formulation	Phosphate buffered saline, pH 7.2 State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Botulinum neurotoxin type B (BoNT/B) is produced by Clostridium botulinum, a genetically diverse class of anaerobic, spore-forming, gram-positive bacilli. Seven different botulinum toxin groups have been identified serologically and are called botulinum toxin type A,B,C,D,E,F, and G. BoNT/B is a two-chain polypeptide with a 100-kDa heavy chain, which is responsible for neurospecific binding joined by a disulphide bond to a 50-kDa light chain, a zinc-endopeptidase which blocks neurotransmitter release. BoNT/B is one of the most poisonous naturally occurring substances. It inhibits acetylcholine release from neuromuscular junctions while it is used as an important therapeutic mainstay in the treatment of spasticity disorders and as a cosmetic treatment.
Synonyms	Botulinum Neurotoxin type B, Bontoxilysin B, Bot B, BoNT/B
Note	SDS-PAGE analysis of GR-3G7: The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Figure.3).
Reference Data

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