Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody [Clone ID: Ks20.8]

Specifications

Product Data
Clone Name	Ks20.8
Applications	IHC
Recommend Dilution	Immunohistochemistry on Paraffin Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytokeratins isolated from human micro dissected villi of human duodenal mucosa.
Specificity	This antibody reacts with cytokeratin polypeptide of 46 kD in immunoblott analyses. Cellular Localization: Cytoplasmic.
Formulation	State: Purified State: Liquid purified Ig fraction containing Sodium Azide as preservative.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	keratin 20
Database Link	Entrez Gene 54474 Human UniProt ID P35900
Background	Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibody is highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colon stomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors, transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas. This antibody is essentially non-reactive in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas. This antibody is often used in conjunction with CK7 and other antibodies to distinguishing colon carcinomas (CK20+), from ovarian, pulmonary, and breast carcinomas.
Synonyms	Cytokeratin 20, Keratin-20, KRT20, CK20, K20, Protein IT
Reference Data

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