CEACAM5 (CEACAM1/5) Mouse Monoclonal Antibody [Clone ID: 4/3/17]

Specifications

Product Data
Clone Name	4/3/17
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400. Flow Cytometry: 1.2 µg/106 cells. Immunohistochemistry on Cryosections: 1-2 µg/106 cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Immunisation with extracted protein of CEACAM5.
Specificity	Recognizes CEACAM1, 5 (BGP, CEA, CD66a/e).
Formulation	PBS, pH 7.2 State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	carcinoembryonic antigen related cell adhesion molecule 5
Database Link	Entrez Gene 1048 Human UniProt ID P06731
Background	CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). It consists of seven CEACAM (CEACAM1, CEACAM3-CEACAM8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immuno-globulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). CEACAM1 expression is down-regulated in many tumors indicating a tumor-suppressive function. The anti-tumor effect may be due to inhibition of tumor angiogenesis, possibly by increased secretion of anti-angiogenic molecules from the cells (3). The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).
Synonyms	Carcinoembryonic antigen, BGP, CEA, CEACAM1, CEACAM5, CD66a,e
Reference Data

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