EGF Mouse Monoclonal Antibody [Clone ID: S-134]

Specifications

Product Data
Clone Name	S-134
Applications	ELISA
Recommend Dilution	ELISA: This antibody can be used as a Capture antibody in sandwich ELISA applications for Human EGF detection in combination with a monoclonal EGF Tracer (AM09149HR-N / AM09149BT-N, Clone S-146). Suggested Capture Coating Dose: 0.3 µg/well; Substrate: TMB. If the above suggested conditions are followed approximately 20 pg/mL of EGF in serum/plasma or 15 pg/mL of EGF in cell medium/urine can be detected with an assay range of 0-1000 pg/mL.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant Human EGF, 6kDa, 53 amino acid residues
Specificity	This antibody reacts with natural and recombinant human EGF. Does not cross react with other Human cytokines tested such as IL-1 beta, IL-8, MCAF, TGF-beta and SAA.
Formulation	0.01M PBS, pH 7.2 without preservatives State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with double distillated water is recommended to adjust the final concentration to 1.00 mg/ml
Concentration	lot specific
Purification	Affinity Chromatograpghy on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	epidermal growth factor
Database Link	Entrez Gene 1950 Human UniProt ID P01133
Background	Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
Synonyms	Urogastrone, Epidermal growth factor, URG, HOMG4
Reference Data
Protein Families	Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
Protein Pathways	Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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