Neurofilament (NEFL) (+ Neurofilament H) Mouse Monoclonal Antibody [Clone ID: 2F11]

Specifications

Product Data
Clone Name	2F11
Applications	IHC, WB
Recommend Dilution	Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Brain.
Reactivity	Feline, Human, Mouse, Opossum, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Neurofilament purified from Human brain.
Specificity	2F11 labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained. Cellular Localization: Cytoplasmic.
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	neurofilament, light polypeptide
Database Link	Entrez Gene 18039 MouseEntrez Gene 83613 RatEntrez Gene 4747 Human UniProt ID P07196
Background	Neurofilaments are the intermediate filaments of neurons and their processes. They are protein triplets composed of three major subunits of MW 68kDa, 155kDa, and 200kDa. Neurofilaments are expressed in tumors of neural origin, or displaying neuronal differentiation, such as neuroblastoma, medulloblastoma, and retinoblastoma.
Synonyms	NF-L,NEFL, NF68, 68 kDa neurofilament protein
Note	Mol. Wt. of Antigen: 68kDa and 200kDa (phosphorylated forms of these subunits).
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways	Amyotrophic lateral sclerosis (ALS)

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