Neurofilament (NEFL) (+ Neurofilament H) Mouse Monoclonal Antibody [Clone ID: 2F11]

Specifications

Product Data
Clone Name	2F11
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/20. Immunohistochemistry on Frozen Sections: 1/160, preferably in PBS. Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200 Pretreatment with TUF is recommended. Positive Control: Brain.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Neurofilament from Human brain.
Specificity	This antibody stains the 70 kD and 200 kD polypeptides of Neurofilament. Not reactive with small cell lung carcinoma. Cross reactivity GFAP: : not detectable Keratin : not detectable Vimentin : not detectable Desmin : not detectable
Formulation	State: Supernatant State: Liquid Supernatant Preservative: 10 mM Sodium Azide, 1% FCS
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	neurofilament, light polypeptide
Database Link	Entrez Gene 4747 Human UniProt ID P07196
Background	Neurofilaments are the intermediate filaments of neurons and their processes. They are protein triplets composed of three major subunits of MW 68kDa, 155kDa, and 200kDa. Neurofilaments are expressed in tumors of neural origin, or displaying neuronal differentiation, such as neuroblastoma, medulloblastoma, and retinoblastoma.
Synonyms	NF-L,NEFL, NF68, 68 kDa neurofilament protein
Note	Mol. Wt. of Antigen: 68kDa and 200kDa (phosphorylated forms of these subunits)
Reference Data

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