Cytokeratin 7 (KRT7) Mouse Monoclonal Antibody [Clone ID: OVTL-12/30]

Specifications

Product Data
Clone Name	OVTL-12/30
Applications	FC, IF, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen and Paraffin Embedded Sections: 1/100- 1/200 with an ABC method. Antibody Cat.-No DM057P is effective on frozen tissue after fixation in Methanol. It is particularly effective on paraffin-embedded tissue, for which pretreatment with e.g. 0.1% pronase / 0.1% trypsine in PBS or 0.5% pepsine in 0.01N HCl or microwave pretreatment is required. Immunocytochemistry. Immunoblotting: 1/100-1/1000. Flow Cytometry: 1/100-1/200.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A cytoskeletal preparation of OTN- 1 ovarian carcinoma cell line
Specificity	This antibody reacts exlcusively with Cytokeratin 7 which is present in specific glandular-type epithelia and most carcinomas derived thereof.
Formulation	PBS containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purifed IgG fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 7
Database Link	Entrez Gene 3855 Human UniProt ID P08729
Background	Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Synonyms	KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin
Reference Data

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