CD49b (ITGA2) Human Monoclonal Antibody [Clone ID: F2H11]

Specifications

Product Data
Clone Name	F2H11
Applications	FC, IF
Recommend Dilution	Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F) . Usage recommendation: *This monoclonal antibody may be used between 5-25μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Human
Host	Human
Clonality	Monoclonal
Immunogen	CD40 activated and EBV immortalized human B lymphocytes
Specificity	HPA5b (does not bind to HPA5a differing by a single amino acid).
Formulation	PBS, 50% glycerol Label: Alexa Fluor 488
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 488
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	integrin subunit alpha 2
Database Link	Entrez Gene 3673 Human UniProt ID P17301
Background	Neonatal AlloImmune Thrombocytopenia (NAIT) is the commonest cause of severe isolated thrombocytopenia in the foetus and newborn. In Cauc asians, 97% of NAIT are caused by HPA-1a or HPA- 5b alloantibodies. By combining CD40 activation and Epstein-Barr virus transformation of B cells isola ted from a woman with high titre of anti -HPA-5b alloan tibodies. F2H11 human monoclonal antibody is highly specific (does not bind to HPA5a) , and allows a r apid and accurate phenotyping of pregnant women as well as selection of blood donors. (Mérieux Y, Rigal D, Pin JJ, XVII th Regional Congress, International Society of Blood Transfusion, Madrid 2007 ; Guillot -Chene P, Lebecque S, Rigal D, Annales Pharmaceutiques Françaises, 06/2009, 67 (3) 182-6).
Synonyms	human platelet antigen 5b
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.