MYD88 Mouse Monoclonal Antibody [Clone ID: 603E10.06]

Specifications

Product Data
Clone Name	603E10.06
Applications	FC, IF
Recommend Dilution	DDX1401P-50 / DDX1401P-100 Purified: FACS intracellular, ImmunoFluorescence. DDX1401A488-50 / DDX1401A488-100 Alexa- fluor®A488: FACS intracellular, ImmunoFluorescence. DDX1401A546-50 / DDX1401A546-100 Alexa- fluor®546: FACS intracellular, ImmunoFluorescence. DDX1401A647-50 / DDX1401A647-100 Alexa- fluor®647: FACS intracellular, ImmunoFluorescence. Applications tested: Intracyto flow cytometry, IF, IHC paraffin. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Canine, Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	huMyd88 transfected 293T cells
Specificity	Human Myd88. Both 603E10.05 and 603E10.06 monoclonal antibodies were validated both in Human and in Dog. 603E10.05 was validated in Swine. Species cross-reactivity: Negative for Murine Myd88.
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol. State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	myeloid differentiation primary response 88
Database Link	Entrez Gene 4615 Human UniProt ID Q99836
Background	MyD88 (myeloid differentiation primary response gene 88) is a universal adapter protein of 296 aa used by all TLRs (except TLR3) and by IL1-R to activate the transcription factor NF-kB. Myd88 binds IRAK1, IRAK2 and TRAF6, leading to NF-kB activation, cytokine secretion and inflammatory response. Myd88 increases IL8 transcription, and is involved in IL18 signaling pathway. A monoclonal antibody was raised against huMyd88-transfected 293T cells. This antibody specifically recognizes endogenously expressed Myd88 and does not cross-react with the mouse Myd88. Both 603E10.05 and 603E10.06 monoclonal antibodies were validated both in human and in dog. 603E10.05 was validated in swine.
Synonyms	Myeloid differentiation primary response protein MyD88
Reference Data

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