alpha Synuclein (SNCA) Mouse Monoclonal Antibody [Clone ID: AS11]

Specifications

Product Data
Clone Name	AS11
Applications	IF
Recommend Dilution	DDX0910P-50 / DDX0910P-100 Purified: ImmunoFluorescence, ImmunoHistoChemistry frozen sections, Western Blot. DDX0910A488-50 / DDX0910A488-100 Alexa-fluor®488: ImmunoFluorescence. DDX0910A546-50 / DDX0910A546-100 Alexa-fluor®546: ImmunoFluorescence. DDX0910A647-50 / DDX0910A647-100 Alexa-fluor®647: ImmunoFluorescence. DDX0910HRP-50 / DDX0910HRP-100 Horseradish Peroxydase: ImmunoHistoChemistry frozen sections, Western Blot. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	rhu α-synuclein (SIGMA).
Specificity	N-terminal portion of Human α-synuclein (aa1-60) Species cross-reactivity: Negative for Murine α-synuclein.
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol. Label: Alexa Fluor 546
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 546
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	synuclein alpha
Database Link	Entrez Gene 6622 Human UniProt ID P37840
Background	α-synuclein (snca) is a140 aas neuronal protein predominantly present in synaptic junctions, specially studied for its relevance in understanding sporadic and familial forms of Parkinson’s disease and related neurodegenerative disorders. In an attempt to break the immunotolerance since the amino-acid sequence is highly conserved between species (95.3% homology between human and mouse), we have immunized C57BL/6 inbred mice presenting a natural deletion for snca with recombinant human α-synuclein and obtained a murine mAb (AS11) recognizing endogenous human α-synuclein. (Kahle et al.,2000, J Neurosci 2000;20:6365–73 ; Specht and Schoepfer, BMC Neurosci 2001;2:11 ; Mougenot AL et al, J Neurosci Methods. 2010 Oct 15;192(2):268-76).
Synonyms	NACP, PARK1
Reference Data

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