IL1 alpha (IL1A) Human Monoclonal Antibody [Clone ID: HuMab X3]

Specifications

Product Data
Clone Name	HuMab X3
Recommend Dilution	DDX0710P-50 / DDX0710P-100 Purified: Inhibition, Neutralization (Binding to IL1α and not to IL1 or IL1Ra, Neutralizing biological effects of natural and recombinant IL1α, Inhibition of IL1α  binding to IL1 receptors. DDX0710A488-50 / DDX0710A488-100 Alexa-fluor®488 (on request). DDX0710A546-50 / DDX0710A546-100 Alexa-fluor®546 (on request). DDX0710A647-50 / DDX0710A647-100 Alexa-fluor®647 (on request).  DDX0710B-50 / DDX0710B-100 Biotin (on request). Usage recommendation: *This monoclonal antibody may be used between 5-25 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Human
Host	Human
Clonality	Monoclonal
Immunogen	CD40 activated and EBV immortalized human B lymphocytes.
Specificity	Human IL1α  (binds to Human IL1α but not to IL1β and IL1Ra).
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol. Label: Alexa Fluor 546
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 546
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	interleukin 1 alpha
Database Link	Entrez Gene 3552 Human UniProt ID P01583
Background	Interleukin-1 (IL-1) defines two polypeptides, IL-1α and IL-1β, that possess a wide spectrum of biological effects. Two natural antagonists of IL-1 action have been characterized: the IL-1 receptor antagonist (IL-1Ra) and a soluble form of the type II IL-1 receptor. Neutralizing autoantibodies to IL-1α have also been detected in sera of healthy individuals and patients with autoimmune or inflammatory diseases. By combining CD40-activation and Epstein-Barr virusimmortalization of B lymphocytes from individuals with circulating anti-IL-1α, a natural anti-IL- 1α monoclonal autoantibody was developed that bound specifically to human IL-1α, but not to IL- 1β and IL-1Ra, with a high affinity (Kd = 1.2 × 10−10 M). HuMAb X3 inhibited IL-1α binding to IL-1 receptors and neutralized biological activities of both recombinant and natural forms of IL-1α. This high affinity neutralizing anti-IL-1α human autoantibody can function as a third type of IL-1 antagonist. (Garrone P et al, Mol Immunol 1996, 33 (7-8), 649-58 ; Guillot-Chene P, Lebecque S, Rigal D, Annales Pharmaceutiques Françaises, 06/2009, 67 (3) 182-6).
Synonyms	IL-1 alpha, IL1F1, IL1A, Hematopoietin-1
Reference Data

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