TLR7 Mouse Monoclonal Antibody [Clone ID: 66H3]

Specifications

Product Data
Clone Name	66H3
Applications	FC, IHC
Recommend Dilution	DDX0500P-50 / DDX0500P-100 Purified: FACS intracellular, ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections. DDX0480A488-50 / DDX0480A488-100 Alexa-fluor®488: FACS intracellular, ImmunoFluorescence.  DDX0500A546-50 / DDX0500A546-100 Alexa-fluor®546: FACS intracellular, ImmunoFluorescence.  DDX0500A647-50 / DDX0500A647-100 Alexa-fluor®647: FACS intracellular, ImmunoFluorescence.  DDX0500B-50 / DDX0500B-100 Biotin: ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections.  Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Canine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant TLR7-transfected eukaryotic cells.
Specificity	Human TLR7.  Species cross-reactivity: Dog
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol. State: Purified
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	toll like receptor 7
Database Link	Entrez Gene 51284 Human UniProt ID Q9NYK1
Background	At least twelve members of the Toll family have been identified. TLR7 is expressed on plasmacytoid DCs, B and T lymphocytes, neutrophils, eosinophils, NK cells, and lung and placenta epithelial cells. TLR7 is localized to endosomal/lysosomal compartments and is reported to respond to single-stranded RNA. Plasmacytoid DCs express TLR7 and respond to activation by producing high amounts of IFN-a / b. TLR7 ligands are R848, Imiquimod (Imidazoquinolin family) and loxoribine (guanosin analog). (Diebold S.S.et al, Science 2004, 303, 1529; Barchet W.A. et al, 2005, Eur J Immunol 3:236; Lund J.M. et al., 2004, PNAS 5598; Hell F. et al., 2004 Science, 303, 1526 ; Gilliet M et al.,2008, Natur Rev Immunol.8, 594).The 66H3 clone was generated through mouse immunization with human TLR7-transfected eukaryotic cells, and was found to cross react with canine TLR7 (Bonnefont C, personal communication).
Synonyms	Toll-like receptor 7
Reference Data

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