IGSF6 Mouse Monoclonal Antibody [Clone ID: 104A10.01]

Specifications

Product Data
Clone Name	104A10.01
Applications	FC, WB
Recommend Dilution	Flow Cytometry surface, Western Blot. Usage recommendation: *This monoclonal antibody may be used between 5-15 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant DORA Ig fusion protein.
Specificity	Human DORA. Species cross-reactivity: Mouse, Canine.
Formulation	Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol. Label: Alexa Fluor 546
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 546
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	immunoglobulin superfamily member 6
Database Link	Entrez Gene 10261 Human UniProt ID O95976
Background	Using a cDNA subtraction technique, a novel member of the immunoglobulin superfamily was isolated from human dendritic cells (DCs). This cDNA, named DORA, for Down-Regulated by Activation encodes a protein belonging to the CD8 family of receptors containing a single V type loop domain with an associated J chain region, a transmembrane region containing an atypical tyrosine residue and a cytoplasmic domain containing three putative tyrosine phosphorylation sites. The human DORA gene has been mapped to chromosome 16. Expression is observed in DCs, purified ex vivo or generated in vitro from either monocytes or CD34+ progenitors, and down-regulated following activation by PMA and Ionomycin or by CD40L engagement. (Bates EE et al, 2000; Immunogenetics, 52: 112-20; Bates EE et al, 1998; Mol. Immunology, 35: 513-24).
Synonyms	DORA
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.