DC SIGN (CD209) Mouse Monoclonal Antibody [Clone ID: 111H2.02]

Specifications

Product Data
Clone Name	111H2.02
Applications	FC
Recommend Dilution	DDX0209P-50 / DDX0209P-100 purified: Surface flow cytometry, IHC Formol- paraffin, Bouin paraffin, Histowax. DDX0209A488-50 / DDX0209A488-100 Alexa-Fluor®488: Surface Flow cytometry. DDX0209A546-50 / DDX0209A546-100 Alexa-Fluor®647: IF.  DDX0209A647-50 / DDX0209A647-100 Alexa-Fluor®647: Surface Flow cytometry.  DDX0209B-50 DDX0209B-100 Biotin (on request): Surface Flow cytometry, IHC.  Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	HeLa cells stably transfected-with human DC-SIGN.
Specificity	Human DC-SIGN. Species cross-reactivity: positive staining on NIH3T3-transfected LSIGN.
Formulation	Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol. Label: Alexa Fluor 488
Concentration	lot specific
Conjugation	Alexa Fluor 488
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	CD209 molecule
Database Link	Entrez Gene 30835 Human UniProt ID Q9NNX6
Background	DC-SIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”)/CD209 and liver/lymph node-specific ICAM-3-grabbing nonintegrin (L-SIGN) (CD299/DC-SIGNR for DC-SIGN-related molecule; DC-SIGN2) are closely related genes that map to chromosome 19p13.3.Both genes encode a member of the C-type lectin family of type II transmembrane proteins. The two receptors are 77% identical at the amino acid level, have similar ligands. They are expressed in different tissues. Both receptors have been shown to interact with ICAM-3 DC-SIGN is a high affinity receptor for HIV gp120 (Soilleux EJ. 2003, Clinical Science 104, 437-; Dakappagari N., et al. 2006, The J Immunol, 176, 426; Geijtenbeeck T.B., et al. 2000, Cell, 100, 57 ; .Bashirova A. et al., 2001, J.Exp. Med., 193, 671). The clone 111H2.02 anti-DC-SIGN blocks HIVgp120-DC-SIGN interaction and reacts on formalin-fixed tissues (Canard B et al, Immunol Lett. 2011, 135(1-2):165-72).
Synonyms	DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.