CLECSF6 (CLEC4A) Mouse Monoclonal Antibody [Clone ID: 111F8.04]

Specifications

Product Data
Clone Name	111F8.04
Applications	FC, IHC, IP, WB
Recommend Dilution	DDX0180P-50 / DDX0180P-100 Purified: FACS surface, ImmunoHistoChemistry frozen sections, Western Blot, Immunoprecipitation. DDX0180A488-50 / DDX0180A488-100 Alexa-fluor®488: FACS surface, ImmunoFluorescence, ImmunoHistoChemistry frozen sections, Western Blot. DDX0180A546-50 / DDX0180A546-100 Alexa-fluor®546: ImmunoFluorescence, Western Blot. DDX0180A647-50 / DDX0180A647-100 Alexa-fluor®647: FACS surface, Western Blot. DDX0180B-50 / DDX0180B-100 Biotin: ImmunoHistoChemistry frozen sections, Western Blot. *This monoclonal antibody may be used between 5-20 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	human DCIR-Ig fusion protein.
Specificity	human DCIR.
Formulation	100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8 State: Purified
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	C-type lectin domain family 4 member A
Database Link	Entrez Gene 50856 Human UniProt ID Q9UMR7
Background	DCIR (dendritic cell immunoreceptor), also known as LLIR, DDB27, CLECSF6, HDCGC13P, is a member of the Dectin-2 family of C-type lectins. DCIR is expressed as a type II membrane glycoprotein of 237 aa with a single carbohydrate recognition domain (CRD), closest in homology to those of the macrophage lectin and hepatic asialoglycoprotein receptors. In contrast to the other members of this family, the intracellular domain of DCIR contains a consensus immunoreceptor tyrosine-based inhibitory motif (ITIM). DCIR is expressed on dendritic cells, monocytes, macrophages, B lymphocytes, neutrophils, granulocytes and plasmacytoid dendritic cells, but not detected on NK and T cells. In vitro, DCIR is strongly expressed by DCs derived from blood monocytes cultured with GM-CSF and IL-4 with a higher expression in CD14+ than CD1a+ derived DC. Finally, DCIR expression is down-regulated by signals inducing DC maturation such as CD40 ligand, LPS, or TNF-. Thus, DCIR is differentially expressed on DCs depending on their origin and stage of maturation/activation. DCIR represents a novel surface molecule expressed by antigenpresenting cells, and of potential importance in regulation of DC function. (Bates EE et al, 1999; J. Immunol., 163: 1973-1983).
Synonyms	C-type lectin DDB27, CLECSF6, DCIR, LLIR, HDCGC13P, Dendritic cell immunoreceptor, Lectin-like immunoreceptor
Reference Data

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