T Cell Receptor (TCR) alpha/beta Hamster Monoclonal Antibody [Clone ID: H57-597]

CAT#: CL075FX

T Cell Receptor (TCR) alpha/beta hamster monoclonal antibody, clone H57-597, FITC



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CNY 5,962.00


货期*
5周

规格
    • 300 ug

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Specifications

Product Data
Clone Name H57-597
Applications FC
Recommend Dilution Flow Cytometry.
Immunohistochemistry on frozen sections.
Reactivity Mouse
Host Hamster
Clonality Monoclonal
Immunogen Affinity-purified DO-11.10 TCR
Donor: Armenian Hamster
Fusion Partner: Mouse myeloma variant P3X63 Ag.653
Specificity This anti-mouse ab T cell receptor monoclonal antibody reacts with the surface of all ab TCR bearing cells and does not react with receptors on gd TCR positive T cells. This monoclonal antibody when used in an immobilized form was able to activate all ab TCR bearing T cell hybridomas tested to produce IL-2. Use of this antibody in conjunction with an anti-CD3e monoclonal antibody allows for accurate measurements of the mutually exclusive sub-populations of ab TCR and gd TCR bearing T cells.
Formulation PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Label: FITC
State: Liquid purified
Concentration lot specific
Purification Protein G Chromatography
Conjugation FITC
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should protected from light.
Synonyms TCRA, TCRB, T-Cell Receptor alpha, T-Cell Receptor beta, T-Cell Receptor alpha beta
Note Protocol: FLOW CYTOMETRY ANALYSIS:

Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add 0.2 - 0.1 µg* of this Ab per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results - Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: BALB/c
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 0.2 µg/10e6 cells
Isotypic Control: FITC Hamster IgG

Cell Source: Percentage of Cells Stained Above Control
Thymus: 76.9% see Figure A
Splenic T Cells: 90.3% see Figure B

Strain Distribution by Flow Cytometry Analysis:
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 1.0 µg/10e6 cells
Strains Tested: C57BL/6, CBA/J, AKR, BALB/c, C3H/He
Positive: C57BL/6, CBA/J, AKR, BALB/c, C3H/He
Negative: none
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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