Neutrophils Rat Monoclonal Antibody [Clone ID: 7/4]

Specifications

Product Data
Clone Name	7/4
Applications	FC, IHC, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen and Paraffin Sections. Western blot. Immunoprecipitation.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Cultured bone marrow cells. Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.
Specificity	This antibody recognises a polymorphic 40kD antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages. Strains positive for clone 7/4: 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains. Negative/Weak for clone 7/4: A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6.
Formulation	PBS without preservatives. State: Low Endotoxin State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at -20°C only. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Note	Endotoxin Level: < 0.01 EU/Ug
Reference Data

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