Sell Rat Monoclonal Antibody [Clone ID: MEL-14]

CAT#: CL032R

Sell rat monoclonal antibody, clone MEL-14, PE



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CNY 3,652.00


货期*
5周

规格
    • 50 ug

Product images

Specifications

Product Data
Clone Name MEL-14
Applications FC
Recommend Dilution Flow Cytometry.
Reactivity Mouse
Host Rat
Clonality Monoclonal
Immunogen Mouse B cell Lymphoma, 38C-14
Specificity This monoclonal antibody reacts with a 90 kDa protein which is involved with the homing of lymphocytes to peripheral lymph nodes.
Formulation PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
Label: PE
State: Liquid purified Ig fraction
Concentration lot specific
Purification Protein G Chromatography
Conjugation PE
Storage Condition Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Gene Name selectin, lymphocyte
Background L-selectin is expressed on most T and B lymphocytes, neutrophils, monocytes, eosinophils.1 Pre-incubation of lymphocytes with this antibody completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro 2,3,6 and the migration of lymphocytes to lymph nodes in vivo.2,3 Polymorphonuclear cells preincubated with this antibody do not migrate to the inflammatory foci.
Synonyms SELL, LNHR, LYAM1, Leu-8, TQ1, gp90-MEL, LECAM1, LAM-1
Note Protocol: FLOW CYTOMETRY ANALYSIS:

Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
4. To each tube, add 0.5-1.0 µg* of this Ab per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive).
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results - Tissue Distribution:
Mouse Strain: BALB/c
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 1.0 µg/10e6 cells
Isotypic Control: PE Rat IgG2a

Cell Source: Percentage of cells stained above control:
Thymus: 89.1%
Spleen: 34.8%
Lymph Node: 88.5%

Results - Strain Distribution:
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 1.0 µg/10e6 cells
Strains Tested: BALB/c, CBA/J, C3H/He, C57BL/6, AKR
Positive: BALB/c, CBA/J, C3H/He, C57BL/6, AKR
Negative: none
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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