Nestin (NES) Mouse Monoclonal Antibody [Clone ID: OTI5F9]

Specifications

Product Data
Clone Name	OTI5F9
Applications	IHC
Recommend Dilution	IHC 1:50
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment of human Nestin(NES) (NP_006608) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Concentration	1mg/ml
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Predicted Protein Size	177.3 kDa
Gene Name	nestin
Database Link	NP_006608 Entrez Gene 10763 Human UniProt ID P48681
Background	This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
Synonyms	Nbla00170
Reference Data
Protein Families	ES Cell Differentiation/IPS

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