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Nestin (NES) (NM_006617) Human Tagged ORF Clone
CAT＃: RC218197
- TrueORF®
NES (Myc-DDK-tagged)-Human nestin (NES)
ORF Plasmid: tGFP
"NM_006617" in other vectors (6)
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CNY 11,088.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Nbla00170 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218197 representing NM_006617
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCTGCATGGGGGAGGAGTCGTTTCAGATGTGGGAGCTCAATCGGCGCCTGGAGGCCTACCTGG CCCGGGTCAAGGCGCTGGAGGAGCAGAATGAGCTGCTCAGCGCGGAGCTCGGGGGGCTCCGGGCACAATC CGCGGACACCTCCTGGCGGGCGCATGCCGACGACGAGCTGGCGGCCCTGCGGGCCCTCGTTGACCAACGC TGGCGGGAGAAGCACGCGGCCGAGGTGGCGCGCGACAACCTGGCTGAAGAGCTGGAGGGCGTGGCAGGCC GATGCCAGCAGCTGCGGCTGGCCCGGGAGCGGACGACGGAGGAGGTAGCCCGCAACCGGCGCGCCGTCGA GGCAGAGAAATGCGCCCGGGCCTGGCTGAGTAGCCAGGTGGCAGAGCTGGAGCGCGAGCTAGAGGCTCTA CGCGTGGCGCACGAGGAGGAGCGCGTCGGCCTGAACGCGCAGGCTGCCTGTGCCCCCCGCTGCCCCGCGC CGCCCCGCGGGCCTCCCGCGCCGGCCCCGGAGGTAGAGGAGCTGGCAAGGCGACTGGGCGAGGCGTGGCG CGGGGCAGTGCGCGGCTACCAGGAGCGCGTGGCACACATGGAGACGTCGCTGGGCCAGGCCCGCGAGCGG CTGGGCCGGGCGGTGCAGGGTGCCCGCGAGGGCCGCCTGGAGCTGCAGCAGCTCCAGGCTGAGCGCGGAG GCCTCCTGGAGCGCAGGGCAGCGTTGGAACAGAGGTTGGAGGGCCGCTGGCAGGAGCGGCTGCGGGCTAC TGAAAAGTTCCAGCTGGCTGTGGAGGCCCTGGAGCAGGAGAAACAGGGCCTACAGAGCCAGATCGCTCAG GTCCTGGAAGGTCGGCAGCAGCTGGCGCACCTCAAGATGTCCCTCAGCCTGGAGGTGGCCACGTACAGGA CCCTCCTGGAGGCTGAGAACTCCCGGCTGCAAACACCTGGCGGTGGCTCCAAGACTTCCCTCAGCTTTCA GGACCCCAAGCTGGAGCTGCAATTCCCTAGGACCCCAGAGGGCCGGCGTCTTGGATCTTTGCTCCCAGTC CTGAGCCCAACTTCCCTCCCCTCACCCTTGCCTGCTACCCTTGAGACACCTGTGCCAGCCTTTCTTAAGA ACCAAGAATTCCTCCAGGCCCGTACCCCTACCTTGGCCAGCACCCCCATCCCCCCCACACCTCAGGCACC CTCTCCTGCTGTAGATGCAGAGATCAGAGCCCAGGATGCTCCTCTCTCTCTGCTCCAGACACAGGGTGGG AGGAAACAGGCTCCAGAGCCCCTGCGGGCTGAAGCCAGGGTGGCCATTCCTGCCAGCGTCCTGCCTGGAC CAGAGGAGCCTGGGGGCCAGCGGCAAGAGGCCAGTACAGGCCAGTCCCCAGAGGACCATGCCTCCTTGGC ACCACCCCTCAGCCCTGACCACTCCAGTTTAGAGGCTAAGGATGGAGAATCCGGTGGGTCTAGAGTGTTC AGCATATGCCGAGGGGAAGGTGAAGGGCAAATCTGGGGGTTGGTAGAGAAAGAAACAGCCATAGAGGGCA AAGTGGTAAGCAGCTTGCAGCAGGAAATATGGGAAGAAGAGGATCTAAACAGGAAGGAAATCCAGGACTC CCAGGTTCCTTTGGAAAAAGAAACCCTGAAGTCTCTGGGAGAGGAGATTCAAGAGTCACTGAAGACTCTG GAAAACCAGAGCCATGAGACACTAGAAAGGGAGAATCAAGAATGTCCGAGGTCTTTAGAAGAAGACTTAG AAACACTAAAAAGTCTAGAAAAGGAAAATAAAGAGCTATTAAAGGATGTGGAGGTAGTGAGACCTCTAGA AAAAGAGGCTGTAGGCCAACTTAAGCCTACAGGAAAAGAGGACACACAGACATTGCAATCCCTGCAAAAG GAGAATCAAGAACTAATGAAATCTCTTGAAGGTAATCTAGAGACATTTTTATTTCCAGGAACGGAAAATC AAGAATTAGTAAGTTCTCTGCAAGAGAACTTAGAGTCATTGACAGCTCTGGAAAAGGAGAATCAAGAGCC ACTGAGATCTCCAGAAGTAGGGGATGAGGAGGCACTGAGACCTCTGACAAAGGAGAATCAGGAACCCCTG AGGTCTCTTGAAGATGAGAACAAAGAGGCCTTTAGATCTCTAGAAAAAGAGAACCAGGAGCCACTGAAGA CTCTAGAAGAAGAGGACCAGAGTATTGTGAGACCTCTAGAAACAGAGAATCACAAATCACTGAGGTCTTT AGAAGAACAGGACCAAGAGACATTGAGAACTCTTGAAAAAGAGACTCAACAGCGACGGAGGTCTCTAGGG GAACAGGATCAGATGACATTAAGACCCCCAGAAAAAGTGGATCTAGAACCACTGAAGTCTCTTGACCAGG AGATAGCTAGACCTCTTGAAAATGAGAATCAAGAGTTCTTAAAGTCACTCAAAGAAGAGAGCGTAGAGGC AGTAAAATCTTTAGAAACAGAGATCCTAGAATCACTGAAGTCTGCGGGACAAGAGAACCTGGAAACACTG AAATCTCCAGAAACTCAAGCACCACTGTGGACTCCAGAAGAAATAAATCAGGGGGCAATGAATCCTCTAG AAAAGGAAATTCAAGAACCACTGGAGTCTGTGGAAGTGAACCAAGAGACATTCAGACTCCTGGAAGAGGA GAATCAGGAATCATTGAGATCTCTGGGAGCATGGAACCTGGAGAATTTGAGATCTCCAGAGGAGGTAGAC AAGGAAAGTCAAAGGAATCTGGAAGAGGAAGAGAACCTGGGAAAGGGAGAGTACCAAGAGTCACTGAGGT CTCTGGAGGAGGAGGGACAGGAGCTGCCGCAGTCTGCAGATGTGCAGAGGTGGGAAGATACGGTGGAGAA GGACCAAGAACTGGCTCAGGAAAGCCCTCCTGGGATGGCTGGAGTGGAAAATGAGGATGAGGCAGAGCTG AATCTGAGGGAGCAGGATGGCTTCACTGGGAAGGAGGAGGTGGTAGAGCAGGGAGAGCTGAATGCCACAG AGGAGGTCTGGATCCCAGGCGAGGGGCACCCAGAGAGCCCTGAGCCCAAAGAGCAGAGAGGCCTGGTTGA GGGAGCCAGTGTGAAGGGAGGGGCTGAGGGCCTCCAGGACCCTGAAGGGCAATCACAACAGGTGGGGGCC CCAGGCCTCCAGGCTCCCCAGGGGCTGCCAGAGGCGATAGAGCCCCTGGTGGAAGATGATGTGGCCCCAG GGGGTGACCAAGCCTCCCCAGAGGTCATGTTGGGGTCAGAGCCTGCCATGGGTGAGTCTGCTGCGGGAGC TGAGCCAGGCCCGGGGCAGGGGGTGGGAGGGCTGGGGGACCCAGGCCATCTGACCAGGGAAGAGGTGATG GAACCACCCCTGGAAGAGGAGAGTTTGGAGGCAAAGAGGGTTCAGGGCTTGGAAGGGCCTAGAAAGGACC TAGAGGAGGCAGGTGGTCTGGGGACAGAGTTCTCCGAGCTGCCTGGGAAGAGCAGAGACCCTTGGGAGCC TCCCAGGGAGGGTAGGGAGGAGTCAGAGGCTGAGGCCCCCAGGGGAGCAGAGGAGGCGTTCCCTGCTGAG ACCCTGGGCCACACTGGAAGTGATGCCCCTTCACCTTGGCCTCTGGGGTCAGAGGAAGCTGAGGAGGATG TACCACCAGTGCTGGTCTCCCCCAGCCCAACGTACACCCCGATCCTGGAAGATGCCCCTGGGCCTCAGCC TCAGGCTGAAGGGAGTCAGGAGGCTAGCTGGGGGGTGCAGGGGAGGGCTGAAGCCCTGGGGAAAGTAGAG AGCGAGCAGGAGGAGTTGGGTTCTGGGGAGATCCCCGAGGGCCCCCAGGAGGAAGGGGAGGAGAGCAGAG AAGAGAGCGAGGAGGATGAGCTCGGGGAGACCCTTCCAGACTCCACTCCCCTGGGCTTCTACCTCAGGTC CCCCACCTCCCCCAGGTGGGACCCCACTGGAGAGCAGAGGCCACCCCCTCAAGGGGAGACTGGAAAGGAG GGCTGGGATCCTGCTGTCCTGGCTTCCGAGGGCCTTGAGGCCCCACCCTCAGAAAAGGAGGAGGGGGAGG AGGGAGAAGAGGAGTGTGGCCGTGACTCTGACCTGTCAGAAGAATTTGAGGACCTGGGGACTGAGGCACC TTTTCTTCCTGGGGTCCCTGGGGAGGTGGCAGAACCTCTGGGCCAGGTGCCCCAGCTGCTACTGGATCCT GCAGCCTGGGATCGAGATGGGGAGTCCGATGGGTTTGCAGATGAGGAAGAAAGTGGGGAGGAGGGAGAGG AGGATCAGGAGGAGGGGAGGGAGCCAGGGGCTGGGCGGTGGGGGCCAGGGTCTTCTGTTGGCAGCCTCCA GGCCCTGAGTAGCTCCCAGAGAGGGGAATTCCTGGAGTCTGATTCTGTGAGTGTCAGTGTCCCCTGGGAT GACAGCTTGAGGGGTGCAGTGGCTGGTGCCCCCAAGACTGCCCTGGAAACGGAGTCCCAGGACAGTGCTG AGCCTTCTGGCTCAGAGGAAGAGTCTGACCCTGTTTCCTTGGAGAGGGAGGACAAAGTCCCTGGCCCTCT AGAGATCCCCAGTGGGATGGAGGATGCAGGCCCAGGGGCAGACATCATTGGTGTTAATGGCCAGGGTCCC AACTTGGAGGGGAAGTCACAGCATGTGAATGGGGGAGTGATGAACGGGCTGGAGCAGTCTGAGGAAGTGG GGCAAGGAATGCCGCTAGTCTCTGAGGGAGACCGAGGGAGCCCCTTTCAGGAGGAGGAGGGGAGTGCTCT GAAGACCTCTTGGGCAGGGGCTCCTGTTCACCTGGGCCAGGGTCAGTTCCTGAAGTTCACTCAGAGGGAA GGAGATAGAGAGTCCTGGTCCTCAGGGGAGGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC218197 representing NM_006617
Red=Cloning site Green=Tags(s) MEGCMGEESFQMWELNRRLEAYLARVKALEEQNELLSAELGGLRAQSADTSWRAHADDELAALRALVDQR WREKHAAEVARDNLAEELEGVAGRCQQLRLARERTTEEVARNRRAVEAEKCARAWLSSQVAELERELEAL RVAHEEERVGLNAQAACAPRCPAPPRGPPAPAPEVEELARRLGEAWRGAVRGYQERVAHMETSLGQARER LGRAVQGAREGRLELQQLQAERGGLLERRAALEQRLEGRWQERLRATEKFQLAVEALEQEKQGLQSQIAQ VLEGRQQLAHLKMSLSLEVATYRTLLEAENSRLQTPGGGSKTSLSFQDPKLELQFPRTPEGRRLGSLLPV LSPTSLPSPLPATLETPVPAFLKNQEFLQARTPTLASTPIPPTPQAPSPAVDAEIRAQDAPLSLLQTQGG RKQAPEPLRAEARVAIPASVLPGPEEPGGQRQEASTGQSPEDHASLAPPLSPDHSSLEAKDGESGGSRVF SICRGEGEGQIWGLVEKETAIEGKVVSSLQQEIWEEEDLNRKEIQDSQVPLEKETLKSLGEEIQESLKTL ENQSHETLERENQECPRSLEEDLETLKSLEKENKELLKDVEVVRPLEKEAVGQLKPTGKEDTQTLQSLQK ENQELMKSLEGNLETFLFPGTENQELVSSLQENLESLTALEKENQEPLRSPEVGDEEALRPLTKENQEPL RSLEDENKEAFRSLEKENQEPLKTLEEEDQSIVRPLETENHKSLRSLEEQDQETLRTLEKETQQRRRSLG EQDQMTLRPPEKVDLEPLKSLDQEIARPLENENQEFLKSLKEESVEAVKSLETEILESLKSAGQENLETL KSPETQAPLWTPEEINQGAMNPLEKEIQEPLESVEVNQETFRLLEEENQESLRSLGAWNLENLRSPEEVD KESQRNLEEEENLGKGEYQESLRSLEEEGQELPQSADVQRWEDTVEKDQELAQESPPGMAGVENEDEAEL NLREQDGFTGKEEVVEQGELNATEEVWIPGEGHPESPEPKEQRGLVEGASVKGGAEGLQDPEGQSQQVGA PGLQAPQGLPEAIEPLVEDDVAPGGDQASPEVMLGSEPAMGESAAGAEPGPGQGVGGLGDPGHLTREEVM EPPLEEESLEAKRVQGLEGPRKDLEEAGGLGTEFSELPGKSRDPWEPPREGREESEAEAPRGAEEAFPAE TLGHTGSDAPSPWPLGSEEAEEDVPPVLVSPSPTYTPILEDAPGPQPQAEGSQEASWGVQGRAEALGKVE SEQEELGSGEIPEGPQEEGEESREESEEDELGETLPDSTPLGFYLRSPTSPRWDPTGEQRPPPQGETGKE GWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLPGVPGEVAEPLGQVPQLLLDP AAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWD DSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGP NLEGKSQHVNGGVMNGLEQSEEVGQGMPLVSEGDRGSPFQEEEGSALKTSWAGAPVHLGQGQFLKFTQRE GDRESWSSGED SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006617 |
| ORF Size | 4863 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006617.2 |
| RefSeq Size | 5591 bp |
| RefSeq ORF | 4866 bp |
| Locus ID | 10763 |
| UniProt ID | P48681 |
| Protein Families | ES Cell Differentiation/IPS |
| MW | 177.3 kDa |
| Gene Summary | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Nestin Expression Affects Resistance to Chemotherapy and Clinical Outcome in Small Cell Lung Cancer
,Sone, K;Maeno, K;Masaki, A;Kunii, E;Takakuwa, O;Kagawa, Y;Takeuchi, A;Fukuda, S;Uemura, T;Fukumitsu, K;Kanemitsu, Y;Ohkubo, H;Takemura, M;Ito, Y;Oguri, T;Inagaki, H;Niimi, A;,
Front. Oncol.
,PubMed ID 32903755
[NES]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218197L1 | Lenti ORF clone of Human nestin (NES), Myc-DDK-tagged |
CNY 13,488.00 |
|
| RC218197L2 | Lenti ORF clone of Human nestin (NES), mGFP tagged |
CNY 18,050.00 |
|
| RC218197L3 | Lenti ORF clone of Human nestin (NES), Myc-DDK-tagged |
CNY 18,050.00 |
|
| RC218197L4 | Lenti ORF clone of Human nestin (NES), mGFP tagged |
CNY 18,050.00 |
|
| RG218197 | NES (tGFP-tagged) - Human nestin (NES) |
CNY 17,770.00 |
|
| SC303801 | NES (untagged)-Human nestin (NES) |
CNY 11,096.00 |
