CD47 Mouse Monoclonal Antibody [Clone ID: OTI3B10]

Specifications

Product Data
Clone Name	OTI3B10
Applications	IHC
Recommend Dilution	IHC 1:7000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD47(NP_001768). The exact sequence is proprietary.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	35.21 kDa
Gene Name	CD47 molecule
Database Link	NP_001768 Entrez Gene 961 Human UniProt ID Q08722
Background	This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
Synonyms	IAP; MER6; OA3
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	ECM-receptor interaction

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