HSP27 (HSPB1) Mouse Monoclonal Antibody [Clone ID: OTI8C7]

Specifications

Product Data
Clone Name	OTI8C7
Applications	WB
Recommend Dilution	WB 1:500-1000
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human HSPB1 (NP_001531) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Predicted Protein Size	22.8 kDa
Gene Name	heat shock protein family B (small) member 1
Database Link	NP_001531 Entrez Gene 3315 Human UniProt ID P04792
Background	The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008].
Synonyms	CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27
Reference Data
Protein Pathways	MAPK signaling pathway, VEGF signaling pathway

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