NG2 (CSPG4) Mouse Monoclonal Antibody [Clone ID: OTI9D5]

Specifications

Product Data
Clone Name	OTI9D5
Applications	WB
Recommend Dilution	WB 1:500
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	250.4 kDa
Gene Name	chondroitin sulfate proteoglycan 4
Database Link	NP_001888 Entrez Gene 1464 Human UniProt ID Q6UVK1
Background	A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]
Synonyms	CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2
Reference Data
Protein Families	Transmembrane

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