IDE Mouse Monoclonal Antibody [Clone ID: OTI4E5]

Specifications

Product Data
Clone Name	OTI4E5
Applications	WB
Recommend Dilution	WB 1:500
Reactivity	Human, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 800-1019 of human IDE (NP_004960) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	117.8 kDa
Gene Name	insulin degrading enzyme
Database Link	NP_004960 Entrez Gene 15925 MouseEntrez Gene 25700 RatEntrez Gene 698640 MonkeyEntrez Gene 3416 Human UniProt ID P14735
Background	This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified. [provided by RefSeq, Sep 2009]
Synonyms	INSULYSIN
Reference Data
Protein Families	Druggable Genome, Protease
Protein Pathways	Alzheimer's disease

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