REV1 Mouse Monoclonal Antibody [Clone ID: OTI1E12]

Specifications

Product Data
Clone Name	OTI1E12
Applications	WB
Recommend Dilution	WB 1:500~2000
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1079-1251 of human REV1 (NP_057400) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	REV1 DNA directed polymerase
Database Link	NP_057400 Entrez Gene 316344 RatEntrez Gene 51455 Human UniProt ID Q9UBZ9
Background	This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]
Synonyms	AIBP80; REV1L
Reference Data
Protein Families	Druggable Genome

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