MLH1 Mouse Monoclonal Antibody [Clone ID: OTI5A7]

Specifications

Product Data
Clone Name	OTI5A7
Applications	WB
Recommend Dilution	WB 1:500
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 299-527 of human MLH1 (NP_000240) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	84.4 kDa
Gene Name	mutL homolog 1
Database Link	NP_000240 Entrez Gene 17350 MouseEntrez Gene 81685 RatEntrez Gene 4292 Human UniProt ID P40692
Background	This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined. [provided by RefSeq, Nov 2009]
Synonyms	COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MMRCS1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer

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