N myc interactor (NMI) Mouse Monoclonal Antibody [Clone ID: OTI2C1]

Specifications

Product Data
Clone Name	OTI2C1
Applications	WB
Recommend Dilution	WB 1:500~2000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-223 of human NMI(NP_004679) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	34.9 kDa
Gene Name	N-myc and STAT interactor
Database Link	NP_004679 Entrez Gene 9111 Human UniProt ID Q13287
Background	NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008]
Synonyms	N-myc (and STAT) interactor; N-myc-interactor; N-myc and STAT interactor; N-myc interactor
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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